Jeong JE, Hong YH. - 41854 N - Int J Dairy Technol 2012 ; in press

Anticancer activity of bovine alpha-lactalbumin treated with microbial transglutaminase.

This study investigated the effects of bovine alpha-lactalbumin (alpha-La) treated with microbial transglutaminase on human cancer cells, cell cultures and growth rate assays. The anticancer activity for 10 mg/mL of bovine alpha-lactalbumin (alpha-La) was measured as ~90% in a human colorectal cancer cell line HCT 116. For the human bone cancer cell line SJSA-1, alpha-La hydrolysis resulted in higher cytotoxicity compared to untreated tumour cells. The formation of polymers of alpha-La was suppressed by the addition of ethylenediaminetetraacetic acid, indicating that polymers of alpha-La are promoted by metal ions such as Ca2+. The effect of alpha-La on the morphology of SJSA-1 cells was manifested as morphological changes compatible with apoptosis. Bovine milk alpha-La with and without microbial transglutaminase is considered a valuable food ingredient and a nutraceutical for human health.